US 7,524,664 B2
	Regio- and enantioselective alkane hydroxylation with modified cytochrome P450
Frances H. Arnold, La Cañada, Calif. (US); Matthew W. Peters, Pasadena, Calif. (US); and Peter Meinhold, Pasadena, Calif. (US)
Assigned to California Institute of Technology, Pasadena, Calif. (US)
Filed on Jun. 15, 2004, as Appl. No. 10/869,825.
Claims priority of provisional application 60/479126, filed on Jun. 17, 2003.
Prior Publication US 2005/0059128 A1, Mar. 17, 2005
Int. Cl. C12N 9/02 (2006.01); C12N 15/00 (2006.01); C12N 1/20 (2006.01); C12P 7/00 (2006.01); C12P 21/04 (2006.01); C07H 21/04 (2006.01); C12Q 1/68 (2006.01); C12Q 1/00 (2006.01); C12Q 1/26 (2006.01)

U.S. Cl. 435—189  [435/4; 435/6; 435/69.1; 435/71.1; 435/25; 435/132; 435/440; 435/252.3; 435/320.1; 536/23.2]

26 Claims

1. An isolated mutant cytochrome P450 Bacillus megaterium 3 (BM-3) enzyme comprising the amino acid sequence of SEQ ID NO:2, except that a leucine is at position 82.