| US 7,524,656 B2 | ||
| Method for producing an L-amino acid by enhancing expression of the yggA gene in an Escherichia bacterium | ||
| Vitaliy Arkadievich Livshits, Moscow (Russian Federation); Natalia Pavlovna Zakataeva, Moscow (Russian Federation); Kazuo Nakanishi, Yokohama (Japan); Vladimir Veniaminovich Aleshin, Moscow (Russian Federation); Petr Vladimirovich Troshin, Moscow (Russian Federation); and Irina Lyvovna Tokhmakova, Moscow (Russian Federation) | ||
| Assigned to Ajinomoto Co., Inc., Tokyo (Japan) | ||
| Filed on Sep. 13, 2007, as Appl. No. 11/854,868. | ||
| Application 11/854868 is a division of application No. 11/276522, filed on Mar. 03, 2006, granted, now 7,399,617, filed on Jul. 15, 2008. | ||
| Application 11/276522 is a division of application No. 11/116286, filed on Apr. 28, 2005, abandoned. | ||
| Application 11/116286 is a continuation of application No. 09/459573, filed on Dec. 13, 1999, granted, now 6,979,560, filed on Dec. 27, 2005. | ||
| Claims priority of application No. 98124016 (RU), filed on Dec. 30, 1998; and application No. 99104431 (RU), filed on Mar. 09, 1999. | ||
| Prior Publication US 2008/0050785 A1, Feb. 28, 2008 | ||
| Int. Cl. C12P 13/04 (2006.01); C12P 13/14 (2006.01); C12P 13/10 (2006.01); C12N 1/21 (2006.01); C12N 15/00 (2006.01); C12P 21/06 (2006.01); C07K 14/00 (2006.01); C07H 21/00 (2006.01) | ||
| U.S. Cl. 435—106 [435/110; 435/114; 435/252.3; 435/252.33; 435/320.1; 435/440; 435/69.1; 530/350; 536/23.1] | 6 Claims |
| 1. A method for producing an L-amino acid selected from the group consisting of L-glutamic acid and L-arginine comprising:
I) cultivating in a culture medium an Escherichia bacterium which has an ability to produce an L-amino acid selected from the group consisting of L-glutamic acid and L-arginine,
wherein said ability to produce the L-amino acid is increased by increasing the expression of a protein comprising the amino
acid sequence shown in SEQ ID NO: 16, wherein the expression is increased relative to the expression of said protein in a
wild-type Escherichia bacterium by increasing the copy number of the DNA coding for said protein or by replacing the native promoter with a stronger
promoter for expression of the DNA coding for said protein, and
II) recovering the L-amino acid from the medium.
|