	US 7,524,648 B2
	Expression system for enhancing solubility and immunogeneicity of recombinant proteins
Ming-Yu Chen, Miaoli (Taiwan); Chin-Kai Chuang, Hsinchu (Taiwan); Yu-Hsyu Su, Miaoli (Taiwan); Chiu-Ting Fan, Hsinchu County (Taiwan); Jung-Chuan Yu, Hsinchu (Taiwan); and Wen-Chuan Lee, Hsinchu (Taiwan)
Assigned to Stresspro Biomedicine Incorporation, Hsinchu (Taiwan); and Animal Technology Institute Taiwan, Miaoli Hsien (Taiwan)
Filed on May 05, 2008, as Appl. No. 12/149,606.
Claims priority of application No. 96115989 A (TW), filed on May 04, 2007.
Prior Publication US 2008/0274508 A1, Nov. 06, 2008
Int. Cl. C12P 21/06 (2006.01); C12N 15/00 (2006.01); C12N 1/20 (2006.01); C07H 21/04 (2006.01)

U.S. Cl. 435—69.1 [435/320.1; 435/252.3; 536/23.1]

20 Claims

1. A chimeric gene comprising:

a. a first DNA sequence encoding a protein transduction domain (PTD) having HIV-Tat PTD activity;

b. a second DNA sequence, linked in translation frame with the first DNA sequence, a encoding J-domain having a heat shock protein 70 (Hsp70)-interacting activity; and

c. a third DNA sequence, linked in translation frame with the second DNA sequence, a target protein or polypeptide.