| US 7,524,640 B2 | ||
| Inhibiting Smad2/3 signaling promotes neurite outgrowth in dorsal root ganglia | ||
| Zhigang He, Boston, Mass. (US); and Fan Wang, Durham, N.C. (US) | ||
| Assigned to Children's Medical Center Corporation, Boston, Mass. (US); and Duke University, Durham, N.C. (US) | ||
| Filed on Aug. 06, 2006, as Appl. No. 11/499,055. | ||
| Prior Publication US 2008/0031911 A1, Feb. 07, 2008 | ||
| Int. Cl. G01N 33/567 (2006.01); C12N 5/06 (2006.01); C12N 5/00 (2006.01); C12N 5/08 (2006.01); C12N 5/16 (2006.01); A01N 1/00 (2006.01) | ||
| U.S. Cl. 435—7.21 [435/368; 435/326; 435/366; 435/1.1] | 1 Claim |
| 1. A method of promoting neurite outgrowth, the method comprising the steps of:
(a) contacting a neuron in situ with an inhibitor of Smad2/3 signaling;
(b) isolating and culturing the neuron; and
(c) detecting a resultant promotion of neurite outgrowth of the neuron in vitro, wherein the neuron is an adult dorsal root
ganglion (DRG) cell and the inhibitor is 2-(5-benzo[1,3]dioxol-5-yl-2-tert-butyl-3H-imidazol-4-yl)-6-methylpyridine hydrochloride
(SB-505124).
|