| US 7,524,637 B2 | ||
| Functional assays that use the T1R1 receptor to screen for T1R1-associated taste modulators | ||
| Jon Elliot Adler, San Diego, Calif. (US); Sergey Zozulya, San Diego, Calif. (US); Xiadong Li, San Diego, Calif. (US); Shawn O'Connell, Encinitas, Calif. (US); and Lena Staszewski, San Diego, Calif. (US) | ||
| Assigned to Senomyx, Inc., San Diego, Calif. (US) | ||
| Filed on Dec. 01, 2003, as Appl. No. 10/724,223. | ||
| Application 10/724223 is a division of application No. 09/799629, filed on Mar. 07, 2001, granted, now 7,244,835. | ||
| Claims priority of provisional application 60/187546, filed on Mar. 07, 2000. | ||
| Claims priority of provisional application 60/195536, filed on Apr. 07, 2000. | ||
| Claims priority of provisional application 60/209840, filed on Jun. 06, 2000. | ||
| Claims priority of provisional application 60/214213, filed on Jun. 23, 2000. | ||
| Claims priority of provisional application 60/226448, filed on Aug. 17, 2000. | ||
| Claims priority of provisional application 60/259227, filed on Jan. 03, 2001. | ||
| Prior Publication US 2004/0171042 A1, Sep. 02, 2004 | ||
| Int. Cl. G01N 33/566 (2006.01); C07K 14/705 (2006.01) | ||
| U.S. Cl. 435—7.2 [435/7.21; 436/501] | 52 Claims |
| 1. A method for identifying a compound that putatively modulates taste in a human subject based on its effect in T1R1 polypeptide
activation comprising:
(i) screening one or more compounds in a functional assay that detects compounds which activate or modulate (enhance or inhibit)
the activation of a human taste receptor comprising a human T1R1 polypeptide selected from the group consisting of:
(a) a T1R1 polypeptide having the amino acid sequence in SEQ. ID. NO: 17;
(b) a human T1R1 polypeptide that possesses at least 90% sequence identity to the polypeptide in SEQ. ID. NO: 17 that specifically
binds to a ligand that also specifically binds to the T1R1 polypeptide in SEQ ID NO: 17;
(c) a human T1R1 polypeptide which is encoded by a nucleic acid sequence that hybridizes to the T1R1 polypeptide coding region
of the nucleic acid sequence in SEQ. ID. NO: 15 or 16 under stringent hybridization conditions which are 50% formamide, 5X
SCC and 1% SDS, incubating at 42 degrees C. and wash in 0.2X SSC and 0.1% SDS at 65 degrees C. and which T1R1 polypeptide
that specifically binds to a taste ligand that specifically binds to the T1R1 polypeptide in SEQ ID NO: 17; and
(ii) identifying compounds that putatively modulate taste in a human subject based on their (a) activation or modulation (inhibition
or enhancement) of the activation of said T1R1 polypeptide according to (a), (b), or (c), in said functional assay (i).
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