US 7,524,494 B2
	Degradation of hydrophobic ester pesticides and toxins
Robyn Joyce Russell, Wanniassa (Australia); Rama Heidari, Amaroo (Australia); Alan Devonshire, Harpenden (United Kingdom); Susan Jane Dorrian, Fraser (Australia); and John Graham Oakeshott, Wanniassa (Australia)
Assigned to Commonwealth Scientific and Industrial Research Organisation, (Australia)
Appl. No. 10/503,643 PCT Filed Feb. 06, 2002, PCT No. PCT/AU02/00114 § 371(c)(1), (2), (4) Date Mar. 23, 2005, PCT Pub. No. WO03/066874, PCT Pub. Date Aug. 14, 2003.
Prior Publication US 2005/0176117 A1, Aug. 11, 2005
Int. Cl. A61K 38/46 (2006.01); C12N 9/18 (2006.01); C12N 1/20 (2006.01); C12N 15/00 (2006.01); C07H 21/04 (2006.01)

U.S. Cl. 424—94.6  [435/197; 435/252.3; 435/320.1; 536/23.2]

26 Claims

1. A purified polypeptide which is a mutant of a Dipteran esterase, wherein the polypeptide comprises a leucine, alanine or valine at residue number 309 of SEQ ID NO: 1, wherein the mutant Dipteran esterase hydrolyzes a hydrophobic ester pesticide or toxin, and wherein the mutant comprises amino acid sequence which is at least 95% identical to SEQ ID NO: 1.