| US 7,521,433 B2 | ||
| Gene carriers with the use of polysaccharide and process for producing the same | ||
| Taro Kimura, Dazaifu (Japan); Masami Mizu, Sakai (Japan); Kazuo Sakurai, Himeji (Japan); Seiji Shinkai, Fukuoka (Japan); and Kazuya Koumoto, Kurume (Japan) | ||
| Assigned to Japan Science and Technology Agency, Tokyo (Japan); Mitsui Sugar Co., Ltd., Saitama (Japan); and Fukuoka Prefectural Government, Fukuoka (Japan) | ||
| Appl. No. 10/471,366 PCT Filed Mar. 11, 2002, PCT No. PCT/JP02/02228 § 371(c)(1), (2), (4) Date Sep. 10, 2003, PCT Pub. No. WO02/072152, PCT Pub. Date Sep. 19, 2002. |
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| Claims priority of application No. 2001-069655 (JP), filed on Mar. 13, 2001; and application No. 2001-130705 (JP), filed on Apr. 27, 2001. | ||
| Prior Publication US 2006/0084149 A1, Apr. 20, 2006 | ||
| Int. Cl. A61K 31/716 (2006.01); C07H 15/04 (2006.01) | ||
| U.S. Cl. 514—54 [536/123.12] | 5 Claims |
1. A gene carrier composed of a complex in which a single-stranded nucleic acid is bound to a double-stranded β-1,3-glucan
wherein the β-1,3-glucan has at least one 1,6-glucopyranoside branch per repeating unit of polysaccharide and is selected
from schizophyllan, lentinan, grifolan, and scleroglucan, and wherein at least some of 1,6-glucopyranoside branches are chemically
modified to be functionalized with nucleic acid-binding functional groups which are cationic functional groups formed by reaction
of the polysaccharide with chain or cyclic compounds containing at least one primary or secondary amino group, wherein the
chemical modification is carried out by periodate-oxidizing the 1,6-glucopyranoside branches and then reductive-aminating
the periodated 1,6-glucopyranoside branches to impart the nucleic acid binding functional group thereto, and wherein the 1,6-glucopyranoside
branch which has been functionalized with the nucleic acid-binding groups is expressed by the following general formula (1):
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