| US 7,517,972 B2 | ||
| Nucleic acid sequences encoding a bitter taste receptor, T2R76 | ||
| Jon Elliot Adler, San Diego, Calif. (US); Huixian Tang, San Diego, Calif. (US); and Alexey Pronin, San Diego, Calif. (US) | ||
| Assigned to Senomyx, Inc., San Diego, Calif. (US) | ||
| Filed on Jul. 29, 2003, as Appl. No. 10/628,464. | ||
| Claims priority of provisional application 60/398727, filed on Jul. 29, 2002. | ||
| Prior Publication US 2004/0132134 A1, Jul. 08, 2004 | ||
| Int. Cl. C07H 21/04 (2006.01); C12N 15/63 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/16 (2006.01); C12N 5/22 (2006.01) | ||
| U.S. Cl. 536—23.5 [435/252.3; 435/254.2; 435/320.1; 435/325; 435/348; 435/364; 435/365; 435/367; 435/369] | 20 Claims |
| 1. An isolated nucleic acid sequence that is selected from the group consisting of:
(i) a nucleic acid sequence that encodes a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID
NO:2 and which specifically binds to a bitter ligand that specifically binds the T2R76 polypeptide of SEQ ID NO:2;
(ii) a nucleic acid sequence that has the sequence of SEQ ID NO:1; and
(iii) a nucleic acid sequence that hybridizes under high stringency conditions to the nucleic acid sequence of SEQ ID NO:1
wherein high stringency conditions are incubating for 15 minutes in 0.1×SSC at 65 degrees C. and which isolated nucleic acid
sequence encodes a taste receptor polypeptide that specifically binds to a bitter ligand that specifically binds to the T2R76
polypeptide of SEQ ID NO:2, and further wherein said isolated nucleic acid sequence of part (i), (ii) or (iii) is operatively
linked to a heterologous promoter that provides for the expression thereof in a recombinant host cell containing said isolated
nucleic acid sequence.
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