	US 7,514,255 B2
	Methods and devices for detecting pancreatic lipase
Stacey Pazar Huth, Yarmouth, Me. (US); and Marilyn I. Strong-Townsend, Yarmouth, Me. (US)
Assigned to IDEXX Laboratories, Inc., Westbrook, Me. (US)
Filed on Dec. 01, 2005, as Appl. No. 11/292,250.
Claims priority of provisional application 60/633114, filed on Dec. 03, 2004.
Prior Publication US 2006/0121549 A1, Jun. 08, 2006
Int. Cl. C12Q 1/34 (2006.01); C12M 1/34 (2006.01)

U.S. Cl. 435—287.1 [435/18]

39 Claims

1. A device for detecting the presence or amount of pancreatic lipase in an animal blood sample, the device being suitable for kit form and capable of being shaken by the user, the device comprising:

(a) a container consisting of a first compartment containing a first liquid reagent comprising a chromogenic lipase substrate, a second compartment containing a second liquid reagent comprising a surface active agent, and an optional third compartment, wherein at least one of the first reagent or the second reagent comprises a colipase, and wherein the container stores the first and the second reagents separately in the first and second compartments, and the container allows for introduction of the sample into the container and the mixing of the sample, the first reagent and the second reagent within the container; and

(b) a system for optically determining the amount of a development of a color change in the mixture of the sample, the first reagent and the second reagent, wherein the system comprises a color chart comprising a gradient of color intensity for visually comparing a color change in a mixture comprising the sample, the first reagent and the second reagent to an amount of pancreatic lipase in the sample.