| US 7,514,225 B2 | ||
| Method for screening an agent that modulates activity of macrophage migration inhibitory factor | ||
| Federico C. A. Gaeta, Mountain View, Calif. (US); Andrew Baird, San Diego, Calif. (US); Jerry Anchin, Del Mar, Calif. (US); Wenbin Ying, San Diego, Calif. (US); Robert Florkiewicz, Seattle, Wash. (US); Jagadish Sircar, San Diego, Calif. (US); and Sunil Kumar K. C., San Diego, Calif. (US) | ||
| Assigned to Avanir Pharmaceuticals, San Diego, Calif. (US) | ||
| Filed on Jul. 15, 2005, as Appl. No. 11/182,360. | ||
| Application 11/182360 is a division of application No. 10/156650, filed on May 24, 2002, granted, now 7,105,519. | ||
| Claims priority of provisional application 60/293642, filed on May 24, 2001. | ||
| Prior Publication US 2005/0287617 A1, Dec. 29, 2005 | ||
| Int. Cl. G01N 33/53 (2006.01); C12N 5/00 (2006.01); C07K 16/24 (2006.01); C07K 14/52 (2006.01) | ||
| U.S. Cl. 435—7.1 [530/351; 530/388.1; 530/389.1; 435/325] | 11 Claims |
| 1. A method for detecting an agent that inhibits the binding of macrophage migration inhibitory factor to an anti-macrophage
migration inhibitor factor monoclonal or polyclonal antibody, comprising:
contacting a test sample containing macrophage migration inhibitory factor produced by THP-1 cells with an agent, and thereafter
with an anti-macrophage migration inhibitory factor monoclonal or polyclonal antibody, wherein the anti-macrophage migration
inhibitory factor monoclonal or polyclonal antibody has a specific binding activity for macrophage inhibitory factor of at
least about 1×105 M−1, and measuring an amount of macrophage migration inhibitory factor in the test sample, wherein an amount of detectable macrophage
migration inhibitory factor is indicative of binding of the anti-macrophage migration inhibitory factor monoclonal or polyclonal
antibody to the macrophage migration inhibitory factor contacted with the agent;
contacting a control sample containing macrophage migration inhibitory factor produced by THP-1 cells with an anti-macrophage
migration inhibitory factor monoclonal or polyclonal antibody in the absence of the agent, and measuring an amount of macrophage
migration inhibitory factor in the control sample, wherein an amount of detectable macrophage migration inhibitory factor
is indicative of binding of the anti-macrophage migration inhibitory factor monoclonal or polyclonal antibody to the macrophage
migration inhibitory factor not contacted with the agent; and
comparing the amount of detectable macrophage migration inhibitory factor in the test sample with the amount of detectable
macrophage migration inhibitory factor in the control sample, wherein a statistically significant decrease in the test sample
amount as compared to the control sample amount is indicative of the agent inhibiting the binding of macrophage migration
inhibitory factor to the anti-macrophage migration inhibitory factor monoclonal or polyclonal antibody.
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