| US 7,514,208 B2 | ||
| Methods for identifying modulators of cyclic nucleotide-gated cation channels | ||
| Christopher D. Creech, Garner, N.C. (US); and Timothy J. Jegla, San Diego, Calif. (US) | ||
| Assigned to ICAgen, Inc., Durham, N.C. (US) | ||
| Filed on Apr. 17, 2007, as Appl. No. 11/736,156. | ||
| Application 11/147623 is a division of application No. 09/927267, filed on Aug. 10, 2001, granted, now 6,933,147. | ||
| Application 11/736156 is a continuation of application No. 11/441293, filed on May 24, 2006, abandoned. | ||
| Application 11/441293 is a continuation of application No. 11/147623, filed on Jun. 07, 2005, granted, now 7,053,183. | ||
| Claims priority of provisional application 60/226253, filed on Aug. 17, 2000. | ||
| Prior Publication US 2008/0145943 A1, Jun. 19, 2008 | ||
| Int. Cl. C12Q 1/00 (2006.01); G01N 33/53 (2006.01); C07K 14/00 (2006.01) | ||
| U.S. Cl. 435—4 [435/7.1; 530/350] | 10 Claims |
| 1. A method for identifying a modulator of a cyclic nucleotide-gated (CNG) cation channel, the method comprising the steps
of:
(i) contacting a compound with a polypeptide, the polypeptide (a) forming, with at least one CNG alpha subunit, a cation channel
having the characteristic of cyclic nucleotide-gating; and (b) comprising the amino acid sequence of SEQ ID NO:1; and
(ii) determining the effect of the compound upon the cation channel, thereby identifying a modulator of the cation channel.
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