	US 7,514,082 B2
	Anti-viral VSF protein and hybridoma producing the same
Yoon-Won Kim, Gangwon-do (Korea, Republic of); Young-Jin Kim, Gangwon-do (Korea, Republic of); Yo-Han Choi, Gyeongsangbuk-do (Korea, Republic of); Jee-Yin Ahn, Gangwon-do (Korea, Republic of); Soo-Dong Woo, Gyeonggi-do (Korea, Republic of); Song-Woo Sin, Gangwon-do (Korea, Republic of); Min-Kee Cho, Gangwon-do (Korea, Republic of); Young-Hwan Byun, Gangwon-do (Korea, Republic of); and Jeung-Yul Kang, Gangwon-do (Korea, Republic of)
Assigned to Immunemed, Inc., Gangwon-do (Korea, Republic of)
Appl. No. 10/500,696 PCT Filed Jan. 30, 2003, PCT No. PCT/KR03/00231 § 371(c)(1), (2), (4) Date Jul. 01, 2004, PCT Pub. No. WO03/064461, PCT Pub. Date Aug. 07, 2003.
Claims priority of application No. 10-2002-0005969 (KR), filed on Feb. 01, 2002.
Prior Publication US 2005/0080236 A1, Apr. 14, 2005
Int. Cl. A61K 39/42 (2006.01); A61K 38/00 (2006.01); A61K 39/00 (2006.01); C12N 5/00 (2006.01); G01N 33/00 (2006.01)

U.S. Cl. 424—159.1 [424/160.1; 424/133.1; 435/325; 436/86; 514/2; 530/808]

14 Claims

1. An isolated virus suppressing factor (VSF) protein having the following properties:

(a) it is increasingly produced in an immune cell stimulated by a variant of encephalomyocarditis virus, EMC-DV;

(b) it has an antiviral activity which is unchanged by immunoprecipitation and immunoneutralization;

(d) it is not one of the group of antiviral cytokines consisting of IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-14, IL-15, IL-16, IL-17, IL-18, G-CSF, GM-CSF, TNF-α, TNF-β, IFN-α, IFN-β, IFN-γ, TGF-β, RANTES, MIP-1α, MIP-1β, MIP-1γ, MCP-1, MCP-3, IP-10 and lymphotactin;

(e) it comprises about 55 kDa polypeptide (H) having the amino acid sequence of SEQ ID NO: 2, about 30 kDa polypeptides (L1 and L2) and about 25 kDa polypeptide (L3) having the amino acid sequence of SEQ ID NO: 4;

(f) it has a molecular weight of over about 100 kDa; and

(g) it is stable at 56° C. for about 40 minutes.