| US 7,510,861 B2 | ||
| Gluconate dehydratase | ||
| Hitoki Miyake, Mobara (Japan); Toshifumi Yamaki, Mobara (Japan); Toshihiro Oikawa, Mobara (Japan); Takeshi Nakamura, Kawasaki (Japan); Hiroki Ishibashi, Omuta (Japan); Yasushi Fukuiri, Omuta (Japan); Atsushi Sakuma, Omuta (Japan); Hironori Komatsu, Sodegaura (Japan); Tomoyuki Ando, Sodegaura (Japan); Kazuhiko Togashi, Sodegaura (Japan); and Hideki Umetani, Sodegaura (Japan) | ||
| Assigned to Mitsui Chemicals, Inc., Tokyo (Japan) | ||
| Filed on Sep. 20, 2006, as Appl. No. 11/523,691. | ||
| Application 11/523691 is a division of application No. 10/885874, filed on Jul. 08, 2004, granted, now 7,125,704. | ||
| Claims priority of application No. 2003-194680 (JP), filed on Jul. 10, 2003. | ||
| Prior Publication US 2007/0015265 A1, Jan. 18, 2007 | ||
| Int. Cl. C12N 9/88 (2006.01); C12P 7/40 (2006.01); C12P 7/58 (2006.01); C12P 21/06 (2006.01) | ||
| U.S. Cl. 435—232 [435/136; 435/137; 435/69.1] | 12 Claims |
| 1. A process for producing a 2-deoxyaldonic acid, comprising the steps of:
reacting in a water based medium an aldonic acid or salt thereof with a gluconate dehydratase capable of dehydrating D-gluconic
acid to produce 2-keto-3-deoxy-D-gluconic acid, thereby converting said aldonic acid or salt thereof into a 2-keto-3-deoxyaldonic
acid; and
reacting in a water based medium said 2-keto-3-deoxyaldonic acid with an oxidizing agent, thereby decarboxylating said 2-keto-3-deoxyaldonic
acid and reducing the carbon number of said 2-keto-3-deoxyaldonic acid by 1 to produce said 2-deoxyaldonic acid; wherein:
said gluconate dehydratase maintains at least 95% of the enzyme activity thereof after being allowed to stand in 30 mM tris(hydroxymethyl)
aminomethane buffer solution with a pH of about 8.5 containing 1 mM of sodium D-gluconate and 1 mM of magnesium chloride at
55° C. for 2 hours; and
said gluconate dehydratase comprises the amino acid sequence shown in SEQ ID NO:2.
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