	US 7,510,833 B2
	Method for examining obesity or leanness
Hidehito Kotani, Tsukuba (Japan); Hiraku Itadani, Tsukuba (Japan); Hiromitsu Araki, Tsukuba (Japan); Kazuhiko Takahashi, Tsukuba (Japan); Satoshi Mashiko, Tsukuba (Japan); Akane Ishihara, Tsukuba (Japan); and Akio Kanatani, Tsukuba (Japan)
Assigned to Banyu Pharmaceutical Co., Ltd., Tokyo (Japan)
Appl. No. 10/548,772 PCT Filed Mar. 12, 2004, PCT No. PCT/JP2004/003452 § 371(c)(1), (2), (4) Date Jun. 19, 2006, PCT Pub. No. WO2004/092368, PCT Pub. Date Oct. 28, 2004.
Claims priority of application No. 2003-067759 (JP), filed on Mar. 13, 2003; and application No. 2003-204354 (JP), filed on Jul. 31, 2003.
Prior Publication US 2007/0031833 A1, Feb. 08, 2007
Int. Cl. C12Q 1/68 (2006.01); C07H 21/04 (2006.01); C12P 19/34 (2006.01)

U.S. Cl. 435—6 [536/24.3; 435/91.2]

1 Claim

1. A method of evaluating body mass in a mouse comprising:

i) obtaining an adipose tissue sample from the mouse;

ii) isolating mRNA from the sample;

iii) analyzing said mRNA to determine the quantity of MCP-1 mRNA of SEQ ID NO. 11; and

iv) determining that said mouse has a propensity for increased body mass if there is at least a 500% increase in the level of MCP-1 mRNA of SEQ ID NO: 11 relative to a control, or a propensity for decreased body mass if there is a least a 70% decrease in the level of MCP-1 mRNA of SEQ ID NO: 11 relative to a control.