US 7,510,706 B2
Pseudotype retroviral vectors containing membrane proteins having hemagglutinin activity
Yoshikazu Yonemitsu, Fukuoka (Japan); Toshihiro Nakajima, Hyogo (Japan); Kenji Nakamaru, Tokyo (Japan); Masanori Kobayashi, Osaka (Japan); Mamoru Hasegawa, Ibaraki (Japan); Yasuji Ueda, Ibaraki (Japan); Akihiro Iida, Ibaraki (Japan); and Hiroyuki Sakakibara, Ibaraki (Japan)
Assigned to DNAVEC Research Inc., Ibaraki (Japan)
Filed on Nov. 29, 2002, as Appl. No. 10/306,949.
Application 10/306949 is a continuation in part of application No. PCT/JP01/04659, filed on Jun. 01, 2001.
Claims priority of application No. 2000-169090 (JP), filed on Jun. 01, 2000.
Prior Publication US 2003/0203489 A1, Oct. 30, 2003
Int. Cl. A01N 63/00 (2006.01); A61K 48/00 (2006.01); C12N 5/00 (2006.01); C12N 15/00 (2006.01); A61K 31/70 (2006.01)
U.S. Cl. 424—93.2  [435/320.1; 435/325; 435/455; 424/93.1; 514/44] 54 Claims
 
1. A composition containing a pseudotype retroviral vector, said composition being free from non-retroviral replicative viruses, wherein the pseudotype retroviral vector comprises:
(a) an HN protein derived from Sendai virus and an amphotropic envelope protein;
(b) an HN protein derived from Sendai virus and a VSV-G protein; or
(c) an HN protein and an F protein each derived from Sendai virus, wherein (i) the HN protein comprises an amino acid sequence derived from a cytoplasmic domain of a retroviral envelope protein at the N-terminus and (ii) a portion or the whole of a cytoplasmic domain of the F protein has been deleted.