US 7,348,167 B2
Increase in the vitamin E content in organisms due to an increase in the tyrosine aminotransferase activity
Ralf Badur, Limburgerhof (Germany); Michael Geiger, Heilbronn (Germany); Rainer Lemke, Quedlinburg (Germany); Klaus-Dieter Salchert, Gernrode (Germany); and Susanne Tropf, Quedlinburg (Germany)
Assigned to SunGene GmbH & Co. KGaA, (Germany)
Appl. No. 10/471,243
PCT Filed Mar. 07, 2002, PCT No. PCT/EP02/02492
§ 371(c)(1), (2), (4) Date Sep. 09, 2003,
PCT Pub. No. WO02/072848, PCT Pub. Date Sep. 19, 2002.
Claims priority of application No. 101 11 676 (DE), filed on Mar. 09, 2001.
Prior Publication US 2004/0086989 A1, May 06, 2004
Int. Cl. C12P 7/26 (2006.01); C12N 9/10 (2006.01); C12N 1/21 (2006.01); C12N 15/74 (2006.01)
U.S. Cl. 435—148  [435/193; 435/471; 435/252.3] 15 Claims
 
1. A process for increasing the production of vitamin E in a vitamin E producing plant, wherein said process comprises expressing a recombinant nucleic acid that encodes a tyrosine aminotransferase which increases tyrosine aminotransferase activity as compared to the wild-type plant, wherein said nucleic acid comprises a sequence selected from the group consisting of:
(a) a polynucleotide sequence encoding a protein comprising the amino acid sequence as set forth in SEQ ID NO: 8, 10, or 12, and
(b) the polynucleotide sequence as set forth in SEQ ID NO: 5;
and growing said recombinant plant, thereby increasing the production of vitamin E in said plant.