| US 7,507,550 B2 | ||
| Analytical sandwich test for determining NT-proBNP | ||
| Juergen Spinke, Lorsch (Germany); Alfons Nichtl, Hohenpeissenberg (Germany); Volker Klemt, Weilheim (Germany); Klaus Hallermayer, Feldafing (Germany); Michael Grol, Feldafing (Germany); Anneliese Borgya, Seeshaupt (Germany); and Andreas Gallusser, Penzberg (Germany) | ||
| Assigned to Roche Diagnostics Operations, Inc., Indianapolis, Ind. (US) | ||
| Filed on Nov. 22, 2004, as Appl. No. 10/994,851. | ||
| Claims priority of application No. 103 55 731 (DE), filed on Nov. 28, 2003. | ||
| Prior Publication US 2005/0118662 A1, Jun. 02, 2005 | ||
| Int. Cl. G01N 33/53 (2006.01); G01N 33/543 (2006.01); G01N 33/544 (2006.01); G01N 33/545 (2006.01); G01N 33/558 (2006.01); G01N 33/577 (2006.01); G01N 33/551 (2006.01); C07K 16/26 (2006.01) | ||
| U.S. Cl. 435—7.5 [435/7.1; 435/7.92; 435/7.94; 435/7.95; 435/70.21; 435/452; 435/336; 435/287.2; 435/287.9; 436/514; 436/518; 436/524; 436/525; 436/528; 436/531; 436/548; 436/811; 530/324; 530/388.24; 530/389.2; 530/391.1; 530/800; 422/55; 422/56; 422/57; 422/58] | 33 Claims |
| 1. An immunological test device for determining N-terminal pro-brain natriuretic peptide (NT-proBNP) comprising:
a sample application zone for receiving a liquid sample;
a monoclonal antibody directed against an epitope of NT-proBNP comprising amino acids 27 to 31, produced by hybridoma cell
line 18.4.34 deposited with DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH under accession number DSM ACC2592,
or a monoclonal antibody directed against an epitope of NT-proBNP comprising amino acids 13 to 16, produced by hybridoma cell
line 17.3.1 deposited with DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH under accession number DSM ACC2591,
a polyclonal antibody selected from polyclonal antibodies directed against epitopes of NT-proBNP comprising amino acids 39
to 50 or amino acids 38 to 42; and
a detection zone in fluid communication with the sample application zone for binding a complex comprising NT-proBNP, the monoclonal
antibody and the polyclonal antibody.
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