| US 7,507,544 B2 | ||
| Functional assays that use the T1R3 receptor to screen for taste modulatory compounds | ||
| Jon Elliot Adler, San Diego, Calif. (US); Sergey Zozulya, San Diego, Calif. (US); Xiadong Li, San Diego, Calif. (US); Shawn O'Connell, Encinitas, Calif. (US); and Lena Staszewski, San Diego, Calif. (US) | ||
| Assigned to Senomyx, Inc., San Diego, Calif. (US) | ||
| Filed on Dec. 02, 2003, as Appl. No. 10/725,081. | ||
| Application 10/725081 is a division of application No. 09/799629, filed on Mar. 07, 2001, granted, now 7,244,835. | ||
| Claims priority of provisional application 60/187546, filed on Mar. 07, 2000. | ||
| Claims priority of provisional application 60/195536, filed on Apr. 07, 2000. | ||
| Claims priority of provisional application 60/209840, filed on Jun. 06, 2000. | ||
| Claims priority of provisional application 60/214213, filed on Jun. 23, 2000. | ||
| Claims priority of provisional application 60/226448, filed on Aug. 17, 2000. | ||
| Claims priority of provisional application 60/259227, filed on Jan. 03, 2001. | ||
| Prior Publication US 2004/0132075 A1, Jul. 08, 2004 | ||
| Int. Cl. G01N 33/566 (2006.01); C07K 14/705 (2006.01) | ||
| U.S. Cl. 435—7.2 [435/7.21; 436/501] | 50 Claims |
| 1. An in vitro method for identifying a compound that putatively elicits or modulates taste in a human subject based on its
effect on T1R3 polypeptide activation comprising:
(1) screening one or more compounds in a functional assay that detects compounds which activate or which modulate (enhance
or inhibit) the activation of a taste receptor comprising a human T1R3 polypeptide or the activation or modulation of said
taste receptor by another compound wherein said T1R3 polypeptide is selected from the group consisting of:
(a) a T1R3 polypeptide having the amino acid sequence of SEQ. ID. NO: 4;
(b) a human T1R3 polypeptide that possesses at least 90% sequence identity to the polypeptide of SEQ. ID. NO: 4 and which
binds to a taste ligand specifically bound by a taste receptor comprising the polypeptide of SEQ ID NO:4; and
(c) a human T1R3 polypeptide which is encoded by a nucleic acid sequence that hybridizes to the complement of the T1R3 polypeptide
coding region of the nucleic acid sequence of SEQ. ID. NO: 2, SEQ ID NO:3 or SEQ. ID. NO: 20 under stringent hybridization
conditions which are incubation in a 50% formamide, 5×SSC and 1% SDS at 42 degrees C. and wash in 0.1% SDS at 65 degrees C.
and which human T1R3 taste receptor binds to a taste ligand that is specifically bound by a taste receptor comprising the
T1R3 polypeptide of SEQ ID NO:4;
(2) identifying compounds (i) that putatively elicit or modulate T1R3 polypeptide-associated taste based on its (a) activation
or modulation (inhibition or enhancement) of the activation of a T1R3 polypeptide by another compound according to (a), (b),
or (c), in said functional assay (1).
|