| US 7,504,250 B2 | ||
| Process for the biological production of 1,3-propanediol with high titer | ||
| Mark Emptage, Wilmington, Del. (US); Sharon L. Haynie, Philadelphia, Pa. (US); Lisa A. Laffend, Claymont, Del. (US); Jeff P. Pucci, Pacifica, Calif. (US); and Gregory Whited, Belmont, Calif. (US) | ||
| Assigned to E. I. du Pont de Nemours and Company, Wilmington, Del. (US) | ||
| Filed on Jan. 16, 2006, as Appl. No. 11/282,497. | ||
| Application 11/282497 is a division of application No. 10/277249, filed on Oct. 21, 2002, granted, now 7,067,300. | ||
| Application 10/277249 is a division of application No. 09/641652, filed on Aug. 18, 2000, granted, now 6,514,733. | ||
| Claims priority of provisional application 60/149534, filed on Aug. 18, 1999. | ||
| Prior Publication US 2006/0148053 A1, Jul. 06, 2006 | ||
| Int. Cl. C12N 1/20 (2006.01); C12N 9/00 (2006.01); C12N 9/02 (2006.01); C12N 15/00 (2006.01); C12N 9/04 (2006.01); C12Q 1/68 (2006.01); C07H 21/04 (2006.01); C12Q 1/00 (2006.01); C12Q 21/04 (2006.01) | ||
| U.S. Cl. 435—252.3 [435/183; 435/189; 435/190; 435/4; 435/6; 435/69.1; 435/71.1; 435/440; 435/320.1; 435/252.33; 435/252.7; 536/23.2] | 9 Claims |
| 1. A recombinant microorganism, useful for the production of 1,3-propanediol, comprising:
(a) at least one gene encoding a glycerol or diol dehydratase α subunit selected from the group consisting of SEQ ID NO:69,
SEQ ID NO:74, SEQ ID NO:79, SEQ ID NO:82, SEQ ID NO:87, SEQ ID NO:91, and SEQ ID NO:96; at least one gene encoding a glycerol
or diol dehydratase β subunit selected from the group consisting of SEQ ID NO:70, SEQ ID NO:75, SEQ ID NO:80, SEQ ID NO:83,
SEQ ID NO:88, SEQ ID NO:92, and SEQ ID NO:97; and at least one gene encoding a glycerol or diol dehydratase γ subunit selected
from the group consisting of SEQ ID NO:71, SEQ ID NO:76, SEQ ID NO:81, SEQ ID NO:84, SEQ ID NO:89, SEQ ID NO:93, and SEQ ID
NO:98;
(b) at least one gene encoding the OrfX polypeptide sequence selected from the group consisting of SEQ ID NO:72, SEQ ID NO:77,
SEQ ID NO:85, SEQ ID NO:94, and SEQ ID NO:99; and at least one gene encoding the OrfZ polypeptide sequence selected from the
group consisting of SEQ ID NO:73, SEQ ID NO:78, SEQ ID NO:86, SEQ ID NO:90, SEQ ID NO:95, and SEQ ID NO:100; and
(c) at least one gene encoding a polypeptide having non-specific catalytic activity to convert 3-hydroxypropionaldehyde to
1,3-propanediol, said polypeptide having the amino acid sequence of SEQ ID NO:57;
wherein, if present, the dhaT gene encoding a 1,3-propanediol oxidoreductase activity has a mutation inactivating the gene
and the microorganism is selected from the group consisting of Citrobacter freundii, Clostridium pasteurianum, Klebsiella pneumoniae, Klebsiella oxytoca, and Escherichia coli, provided that, when the microorganism is Citrobacter freundii, Clostridium pasteurianum, Klebsiella pneumoniae, or Klebsiella oxytoca the at least one gene of (c) is an exogenous gene.
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