| US 7,501,500 B2 | ||
| Inhibiting denaturation of nucleic acid duplexes | ||
| Neil Butt, Cambridge (United Kingdom); Matthew Baker, Cambridge (United Kingdom); and Navin Deepal Pathirana, Cape Elizabeth, Me. (US) | ||
| Assigned to Whatman, Inc., Florham Park, N.J. (US) | ||
| Filed on Jun. 05, 2007, as Appl. No. 11/810,593. | ||
| Application 11/810593 is a division of application No. 10/276886, granted, now 7,244,561, previously published as PCT/GB01/02564, filed on Jun. 11, 2001. | ||
| Claims priority of application No. 0014324.8 (GB), filed on Jun. 12, 2000. | ||
| Prior Publication US 2007/0281316 A1, Dec. 06, 2007 | ||
| Int. Cl. C07H 19/00 (2006.01); C07H 21/04 (2006.01); C07K 14/00 (2006.01) | ||
| U.S. Cl. 536—22.1 [536/23.1; 530/350] | 18 Claims |
| 1. A kit for isolating nucleic acid from a sample comprising cells containing nucleic acid, comprising: (a) a filter supported
by a support; (b) a solution having a pH of from 3 to 11 and comprising (1) a soluble protein or mixture of proteins and (2)
0.1 mM to 10 mM divalent cations, wherein:
(a) the nature and concentration of the protein or mixture of proteins is selected so that the solution is capable of inhibiting
heat denaturation of a nucleic acid duplex; and
(b) the protein or mixture of proteins comprises whole fetal calf blood serum or whole adult bovine serum.
|