	US 7,501,290 B2
	Intraplexing method for improving precision of suspended microarray assays
Brian P. Hanley, 300 Atrium Way #312, Davis, Calif. 95616 (US)
Filed on Sep. 13, 2007, as Appl. No. 11/901,117.
Claims priority of provisional application 60/844577, filed on Sep. 13, 2006.
Prior Publication US 2008/0064122 A1, Mar. 13, 2008
Int. Cl. G01N 33/543 (2006.01)

U.S. Cl. 436—518

17 Claims

1. A method for improving the statistical significance of readings from a single sample, the method comprising:

a. performing a first assay on a microparticle media for reading by an instrument, the first assay comprising;

i. m number of SMPCS-IDG assays targeted at a common analyte;

ii. wherein each said SMPCS-IDG assay is designed to exhibit a different response level to said common analyte;

iii. wherein each said SMPCS-IDG assay comprises n number of SMPCS assays;

iv. wherein each said SMPCS assay is designed to exhibit a substantially identical response level to said common analyte;

v. wherein m is an integer of at least 1 and n is an integer of at least 1;

vi. wherein if m equals 1 then n is greater than 1 and if n equals 1 then m is greater than 1; and

vii. wherein m and n are not required to be the same value and n is not necessarily the same value for each m; and

b. obtaining an SMPCS reading from each said SMPCS assay in each said SMPCS-IDG;

i. wherein there are n number of SMPCS readings for each of said m number of SMPCS-IDG assays.