	US 7,501,257 B2
	Molecular diagnostic method of a cancer tissue or a cancer cell
Toshiyuki Sakai, Kyoto (Japan); and Hideki Ishihara, Mino-gun (Japan)
Assigned to Sysmex Corporation, Kobe-shi, Hyogo (Japan)
Appl. No. 10/547,072 PCT Filed Feb. 25, 2004, PCT No. PCT/JP2004/002164 § 371(c)(1), (2), (4) Date Oct. 27, 2005, PCT Pub. No. WO2004/076686, PCT Pub. Date Sep. 10, 2004.
Claims priority of application No. 2003-048653 (JP), filed on Feb. 26, 2003.
Prior Publication US 2007/0031813 A1, Feb. 08, 2007
Int. Cl. C12Q 1/48 (2006.01)

U.S. Cl. 435—15

14 Claims

1. A method of diagnosing a cell for cancer comprising:

measuring a first activity value of CDK1 and a second activity value of CDK2 in a cell collected from a patient;

obtaining a first relative activity value represented by a ratio of the second activity value to the first activity value;

producing a cell cycle profile on the basis of the obtained first relative activity value;

comparing the produced cell cycle profile with a cell cycle profile of a normal cell; and

determining that the cell collected from the patient is a cancer cell, when a statistically significant difference between the produced cell cycle profile and the cell cycle profile of a normal cell exists.