	US 7,501,253 B2
	DNA fingerprinting using a branch migration assay
Nader Pourmand, San Mateo, Calif. (US); Ronald W. Davis, Palo Alto, Calif. (US); and Shan X. Wang, Portola Valley, Calif. (US)
Assigned to The Board of Trustees of the Leland Stanford Junior University, Palo Alto, Calif. (US)
Filed on Apr. 24, 2007, as Appl. No. 11/789,559.
Application 11/789559 is a continuation in part of application No. 11/231657, filed on Sep. 20, 2005, granted, now 7,238,486.
Claims priority of provisional application 60/612000, filed on Sep. 21, 2004.
Prior Publication US 2007/0287156 A1, Dec. 13, 2007
Int. Cl. C12Q 1/68 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01)

U.S. Cl. 435—6 [536/23.1; 536/24.3]

15 Claims

1. A method of determining the length of a polynucleotide target, comprising:

(a) hybridizing said polynucleotide target to an array of first probes having different, determined lengths to form duplexes between said polynucleotide target and said first probes;

(b) hybridizing a second probe having a determined length to said duplexes, wherein said second probe displaces said polynucleotide target from said duplex if the length of said polynucleotide target is greater than the length of said first probe;

(c) hybridizing a third probe having a determined length to said duplexes, wherein said third probe displaces said polynucleotide target from said duplex if the length of said polynucleotide target is less than the length of said first probe;

(d) determining the length of said polynucleotide target by identifying in which of said duplexes said polynucleotide target was not displaced.