US 7,498,483 B2
Phospholipid:diacylglycerol acyltransferases
Karlene H. Butler, Newark, Del. (US); Rebecca E. Cahoon, Webster Grove, Mo. (US); Omolayo O. Famodu, Newark, Del. (US); Sarah E. Hall, Thorndale, Pa. (US); and Edgar B. Cahoon, Webster Grove, Mo. (US)
Assigned to E. I. du Pont de Nemours and Company, Wilmington, Del. (US)
Filed on Oct. 31, 2007, as Appl. No. 11/930,384.
Application 11/930384 is a division of application No. 11/315766, filed on Dec. 22, 2005, granted, now 7,326,828.
Application 11/315766 is a division of application No. 10/321802, filed on Dec. 17, 2002, granted, now 7,053,269.
Claims priority of provisional application 60/341448, filed on Dec. 17, 2001.
Prior Publication US 2008/0057585 A1, Mar. 06, 2008
Int. Cl. A01H 1/00 (2006.01); C07H 21/04 (2006.01); C12N 15/29 (2006.01); C12N 15/52 (2006.01); C07K 14/415 (2006.01)
U.S. Cl. 800—295  [435/6; 435/69.1; 435/468; 435/419; 435/252.3; 435/320.1; 435/183; 530/370; 536/23.6; 800/278] 11 Claims
 
1. An isolated polynucleotide comprising:
(a) a nucleotide sequence encoding a polypeptide having phospholipid:diacylglycerol acyltransferase activity, wherein the polypeptide has an amino acid sequence of at least 90% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO: 4, or
(b) a complement of the nucleotide sequence, wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.