US 7,498,143 B2
	Identification of Actinobacillus actinomycetemcomitans antigens for use in the diagnosis, treatment, and monitoring of periodontal diseases
Martin Handfield, Gainesville, Fla. (US); Jeffrey Daniel Hillman, Gainesville, Fla. (US); and Ann Progulske-Fox, Keystone Heights, Fla. (US)
Assigned to University of Florida Research Foundation, Gainesville, Fla. (US)
Filed on Jan. 17, 2006, as Appl. No. 11/333,747.
Application 11/333747 is a division of application No. 09/995493, filed on Nov. 28, 2001, granted, now 7,052,860.
Application 09/995493 is a continuation in part of application No. PCT/US02/37235, filed on Nov. 20, 2002.
Application PCT/US02/37235 is a continuation in part of application No. PCT/US03/32645, filed on Oct. 15, 2003.
Prior Publication US 2006/0160159 A1, Jul. 20, 2006
Int. Cl. G01N 33/554 (2006.01); G01N 33/569 (2006.01); G01N 33/53 (2006.01)

U.S. Cl. 435—7.32  [435/7.2; 435/7.72; 435/7.92; 435/7.93; 435/7.94]

13 Claims

1. A method of detecting the presence of A. actinomycetemcomitans or an A. actinomycetemcomitans antigen in a test sample comprising contacting the test sample with an antibody or a fragment thereof, wherein the fragment is a Fab, Fab′, Fab′-SH, F(ab′)2, or Fv fragment, that specifically binds to a polypeptide consisting of SEQ ID NO:146, and detecting an immunocomplex comprising A. actinomycetemcomitans or an A. actinomycetemcomitans antigen and the antibody or fragment thereof, wherein detection of the immunocomplex indicates the presence of A. actinomycetemcomitans or an A. actinomycetemcomitans antigen in the test sample.