| US 7,494,820 B2 | ||
| Electrochemiluminescence of rare earth metal chelates | ||
| Hongjun Yang, Rockville, Md. (US); and Nicholas Cairns, Gaithersburg, Md. (US) | ||
| Assigned to Bioveris Corporation, Gaithersburg, Md. (US) | ||
| Filed on Mar. 11, 2005, as Appl. No. 11/77,076. | ||
| Application 09/222443 is a division of application No. 08/891337, filed on Jul. 10, 1997, granted, now 5,858,676. | ||
| Application 11/077076 is a continuation of application No. 09/222443, filed on Dec. 29, 1998, abandoned. | ||
| Application 08/891337 is a continuation of application No. 08/423394, filed on Apr. 18, 1995, abandoned. | ||
| Prior Publication US 2005/0176055 A1, Aug. 11, 2005 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. G01N 21/76 (2006.01); G01N 33/532 (2006.01); G01N 33/533 (2006.01); G01N 33/24 (2006.01); C07K 1/10 (2006.01) | ||
| U.S. Cl. 436—172 [435/7.92; 435/7.93; 435/7.94; 435/7.95; 436/544; 436/546; 436/172; 436/56; 436/81; 530/402; 530/406] | 18 Claims |
| 1. A method of determining the presence of an analyte of interest which binds to a chemical moiety, which comprises
(a) forming a reagent mixture comprising the chemical moiety and a sample that may contain the analyte of interest; such that
the chemical moiety and the analyte specifically bind to one another, said chemical moiety having the formula
[MPL1L2-(-link-)-]tB
wherein M is a lanthanide;
P is a polydentate ligand of M;
L1 and L2 are ligands of M, each of which may be a substance covalently bound to one or more of P, L1 or L2 through one or more covalent bond linkages, said linkages designated as (-link-) and being covalent bonds linking B with at
least one of P, L1 or L2;
t is an integer equal to or greater than 1;
B is a biological substance or a synthetic substance which is capable of specifically binding to the analyte of interest;
P, L1, L2, and B are of such number that the total number of bonds to M provided by the ligands of M equals the coordination number
of M; and
P, L1, L2, and B are of such composition that the chemical moiety can be induced to repeatedly emit electromagnetic radiation;
(b) exposing the reagent mixture to electrochemical energy, the potential of which oscillates between a potential sufficiently
positive and sufficiently negative to induce the chemical moiety to oscillate between at least two oxidization states, thereby
inducing the chemical moiety to repeatedly electrochemiluminesce; and
(c) detecting emitted luminescence thereby to determine the presence of the analyte of interest.
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