US 7,494,786 B2
	Mutant proteinase with reduced self-cleavage activity and method of purification
Jennifer A. Doudna, Berkeley, Calif. (US); Louise J. Lucast, Guilford, Conn. (US); and Robert T. Batey, Boulder, Colo. (US)
Assigned to Yale University, New Haven, Conn. (US)
Appl. No. 10/343,766 PCT Filed Jun. 11, 2001, PCT No. PCT/US01/18620 § 371(c)(1), (2), (4) Date Oct. 09, 2003, PCT Pub. No. WO01/96539, PCT Pub. Date Dec. 20, 2001.
Claims priority of provisional application 60/211535, filed on Jun. 15, 2000.
Prior Publication US 2004/0072179 A1, Apr. 15, 2004
Int. Cl. C12P 21/06 (2006.01); C12P 21/02 (2006.01); C12N 15/00 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 9/50 (2006.01); C07H 21/04 (2006.01)

U.S. Cl. 435—69.1  [435/320.1; 435/252.3; 435/219; 435/325; 536/23.1]

23 Claims

1. An isolated active mutant tobacco etch virus (TEV) proteinase having a molecular weight of about 27 kDa and reduced self-cleavage activity relative to the self-cleavage activity of wild-type TEV proteinase.