| US 7,332,648 B2 | ||
| Unactivated oocytes in nuclear transfer to produce cultured inner cell mass cells and ungulates | ||
| Keith Henry Stockman Campbell, Midlothian (United Kingdom); and Ian Wilmut, Midlothian (United Kingdom) | ||
| Assigned to Roslin Institute, Midlothian (United Kingdom) | ||
| Filed on Nov. 21, 2001, as Appl. No. 9/989,126. | ||
| Application 09/989126 is a continuation of application No. 09/650285, filed on Aug. 29, 2000, granted, now 6,525,243. | ||
| Application 09/650285 is a continuation of application No. 08/803165, filed on Feb. 19, 1997, granted, now 6,252,133. | ||
| Claims priority of application No. 9517779.6 (GB), filed on Aug. 31, 1995; and application No. PCT/GB96/02098 (WO), filed on Aug. 30, 1996. | ||
| Prior Publication US 2002/0112254 A1, Aug. 15, 2002 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. C12N 15/00 (2006.01); C12N 5/08 (2006.01) | ||
| U.S. Cl. 800—24 [435/366; 435/377] | 37 Claims |
| 1. A method for producing a mammalian cultured inner cell mass cell by nuclear transfer comprising:
(i) inserting a nucleus of a diploid non-human mammalian differentiated somatic cell in the G1 phase of the cell cycle into an unactivated, enucleated metaphase II-arrested non-human mammalian oocyte of the same species
to reconstruct an embryo;
(ii) activating the resultant reconstructed embryo;
(iii) culturing said activated, reconstructed embryo; and
(iv) isolating and culturing inner cell mass cells obtained from said cultured activated, reconstructed embryo to obtain a
cultured inner cell mass cell.
|