| US 7,332,355 B2 | ||
| Method and compositions for the detection of protein glycosylation | ||
| Linda Hsieh-Wilson, San Marino, Calif. (US); Nelly Khidekel, Pasadena, Calif. (US); Hwan-Ching Tai, Pasadena, Calif. (US); and Sabine Arndt, Dortmund (Germany) | ||
| Assigned to California Institute of Technology, Pasadena, Calif. (US) | ||
| Filed on Nov. 17, 2004, as Appl. No. 10/990,767. | ||
| Claims priority of provisional application 60/523523, filed on Nov. 18, 2003. | ||
| Prior Publication US 2005/0130235 A1, Jun. 16, 2005 | ||
| Int. Cl. G01N 33/532 (2006.01); G01N 33/53 (2006.01); G01N 33/573 (2006.01); G01N 33/533 (2006.01); C07K 1/13 (2006.01); C12Q 7/58 (2006.01); C12Q 1/54 (2006.01) | ||
| U.S. Cl. 436—544 [436/545; 436/546; 436/172; 435/7.1; 435/7.4; 435/7.75; 435/7.6; 435/14; 435/137; 530/402; 530/403] | 26 Claims |
| 1. A method for detecting a post-translationally modified protein with a glycosyl group comprising
contacting the protein with a glycosyl transferase enzyme and a labeling agent, wherein the labeling agent comprises a chemical
handle and a transferable glycosyl group, wherein the glycosyl transferase catalyzes the transfer of the labeling agent and
the chemical handle to the post-translationally modified protein;
contacting the modified protein having the transferred labeling agent and the chemical handle with a detection agent, wherein
the chemical handle reacts with a reactive group on the detection agent to form a covalent bond; and
detecting the detection agent, that is covalently bound to said chemical handle
wherein the detection of the bound detection agent indicates the presence of a post-translationally modified protein.
|