| US 7,332,311 B2 | ||
| Fatty acyl-CoA: fatty alcohol acyltransferases | ||
| Kathryn D. Lardizabal, Woodland, Calif. (US); James G. Metz, Longmont, Colo. (US); and Michael W. Lassner, Foster City, Calif. (US) | ||
| Assigned to Calgene LLC, Davis, Calif. (US) | ||
| Filed on Apr. 18, 2003, as Appl. No. 10/418,146. | ||
| Application 10/418146 is a continuation of application No. 09/454685, filed on Dec. 03, 1999, abandoned. | ||
| Application 09/454685 is a continuation in part of application No. 09/205815, filed on Dec. 04, 1998, granted, now 6,492,509. | ||
| Application 09/205815 is a continuation in part of application No. 09/092562, filed on Jun. 05, 1998, granted, now 6,596,538. | ||
| Application 09/092562 is a continuation of application No. 08/265047, filed on Jun. 23, 1994, granted, now 5,679,881. | ||
| Claims priority of provisional application 60/048651, filed on Jun. 05, 1997. | ||
| Prior Publication US 2003/0228668 A1, Dec. 11, 2003 | ||
| Int. Cl. C12P 7/64 (2006.01); C12P 21/06 (2006.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01); C12N 5/10 (2006.01); C12N 5/14 (2006.01); C12N 1/21 (2006.01); C07K 14/00 (2006.01) | ||
| U.S. Cl. 435—134 [435/69.1; 435/325; 435/252.3; 435/410; 435/191; 435/193; 536/23.2; 536/23.6; 530/350] | 13 Claims |
| 1. A method of producing a wax ester in a host cell comprising the steps of
growing a host cell having a recombinant nucleic acid construct comprising a promoter functional in said host cell and a nucleic
acid sequence encoding a wax synthase, wherein the amino acid sequence of said wax synthase is SEQ ID NO: 2 and said host
cell comprises an endogenous fatty acyl-CoA reductase and a β-ketoacyl-CoA synthase;
and obtaining expression of said nucleic acid encoding said wax synthase, wherein said host cell comprises a fatty alcohol
substrate of said wax synthase, and said wax ester is produced in said host cell.
|