US 7,491,531 B2
Randomized libraries of zinc finger proteins
Casey C. Case, San Mateo, Calif. (US); Edward J. Rebar, El Cerrito, Calif. (US); and Andrew Jamieson, San Francisco, Calif. (US)
Assigned to Sangamo BioSciences, Inc., Richmond, Calif. (US)
Filed on Jan. 06, 2003, as Appl. No. 10/337,216.
Application 10/337216 is a continuation of application No. 09/731558, filed on Dec. 06, 2000, granted, now 6,503,717.
Application 09/731558 is a continuation in part of application No. 09/456100, filed on Dec. 06, 1999, abandoned.
Prior Publication US 2003/0134318 A1, Jul. 17, 2003
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/11 (2006.01); C12N 15/63 (2006.01); C12N 15/79 (2006.01)
U.S. Cl. 435—325  [435/419; 435/320.1] 10 Claims
 
1. A nucleic acid library encoding randomized zinc finger proteins, wherein the library is generated by recognition helix grafting or by gene shuffling.