| US 7,491,528 B2 | ||
| Method for extracellular production of target proteins employing OmpF in E. coli | ||
| Sang-Yup Lee, Taejon (Korea, Republic of); and Ki-Jun Jeong, Taejon (Korea, Republic of) | ||
| Assigned to Korea Advanced Institute of Science and Technology, (Korea, Republic of) | ||
| Filed on Jun. 19, 2003, as Appl. No. 10/600,145. | ||
| Application 10/600145 is a continuation of application No. PCT/KR02/01547, filed on Aug. 13, 2002. | ||
| Claims priority of application No. 10-2001-0048881 (KR), filed on Aug. 14, 2001. | ||
| Prior Publication US 2005/0019857 A1, Jan. 27, 2005 | ||
| Int. Cl. C12P 21/06 (2006.01); C12P 21/04 (2006.01); C12N 15/64 (2006.01); C12N 1/20 (2006.01); C12N 15/00 (2006.01); C12N 15/09 (2006.01); C12N 15/63 (2006.01); C12N 15/70 (2006.01); C12N 15/74 (2006.01) | ||
| U.S. Cl. 435—320.1 [435/69.1; 435/69.7; 435/71.1; 435/71.2; 435/91.4; 435/252.3; 435/252.33; 435/471; 435/476] | 26 Claims |
| 1. An expression vector comprising:
an OmpF promoter;
an OmpF gene encoding an OmpF protein:
a cleavage-site gene encoding an RNA or protein cleavage site; and
a gene of interest encoding a protein of interest,
wherein the expression vector encodes a fusion protein comprising the OmpF protein, the cleavage site and the protein of interest,
and wherein the cleavage-site gene is located between the OmpF gene and the gene of interest in the expression vector such
that the RNA or protein cleavage site is located between the OmpF protein and the protein of interest in the fusion protein.
|