	US 7,491,506 B2
	Inhibition of B-cell maturation and antibody production
Michael Karin, La Jolla, Calif. (US); Uwe Senftleben, Obereldingen (Germany); and Yixue Cao, San Diego, Calif. (US)
Assigned to The Regents Of The University Of California, Oakland, Calif. (US)
Filed on Jan. 11, 2006, as Appl. No. 11/330,582.
Application 11/330582 is a continuation of application No. 10/176789, filed on Jun. 21, 2002, abandoned.
Claims priority of provisional application 60/300109, filed on Jun. 21, 2001.
Prior Publication US 2006/0105414 A1, May 18, 2006
Int. Cl. C12Q 1/48 (2006.01)

U.S. Cl. 435—15

12 Claims

1. A method of screening a compound for reducing development of humoral immunity, comprising

a) providing:

i) B lymphocytes comprising IκB kinaseα (IKKα) comprising a sequence set forth in SEQ ID NO:1; and

ii) a compound to be screened;

b) contacting said B lymphocytes with said compound;

c) detecting a reduced level of IKKα protein kinase activity in said B lymphocytes in the presence of said compound, thereby identifying said test compound as reducing development of humoral immunity.