| US 7,491,495 B2 | ||
| Adsorption of nucleic acids to a solid phase | ||
| Ralf Zielenski, Benediktbeuern (Germany); Klaus Geissler, Oberhausen (Germany); and Thomas Walter, Penzberg (Germany) | ||
| Assigned to Roche Diagnostics Operations, Inc., Indianapolis, Ind. (US) | ||
| Filed on Feb. 18, 2005, as Appl. No. 11/61,948. | ||
| Claims priority of application No. 04003898 (EP), filed on Feb. 20, 2004. | ||
| Prior Publication US 2005/0214926 A1, Sep. 29, 2005 | ||
| Int. Cl. C12Q 1/68 (2006.01); C07H 19/00 (2006.01); C07H 21/00 (2006.01) | ||
| U.S. Cl. 435—6 [536/22.1; 536/25.4] | 19 Claims |
| 1. A method for adsorbing a nucleic acid from a biological sample to a solid phase, comprising the steps of
providing an aqueous lysis buffer that contains a chaotropic agent;
mixing the lysis buffer with the biological sample, whereby the concentration of the chaotropic agent in the mixture is between
1 M and 4 M, and incubating the mixture;
dissolving an additional amount of chaotropic agent in or adding an aqueous solution containing additional chaotropic agent
to the mixture of step (b), thereby increasing the concentration of the chaotropic agent in the mixture by more than 0.5 M;
and
contacting the mixture of step (c) with the solid phase, thereby adsorbing the nucleic acid from the mixture to the solid
phase,
wherein alcohol is not used to effectuate the adsorption.
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