| US 7,491,311 B2 | ||
| Nucleic acid detection sensor | ||
| Koji Hashimoto, Sagamihara (Japan); Hirohisa Miyamoto, Kamakura (Japan); Kazuhiro Itsumi, Kawasaki (Japan); and Kouhei Suzuki, Yokohama (Japan) | ||
| Assigned to Kabushiki Kaisha Toshiba, Tokyo (Japan) | ||
| Filed on Oct. 05, 2004, as Appl. No. 10/957,700. | ||
| Application 10/957700 is a division of application No. 09/961249, filed on Sep. 25, 2001, granted, now 6,818,109. | ||
| Claims priority of application No. 2000-301516 (JP), filed on Sep. 29, 2000. | ||
| Prior Publication US 2005/0048558 A1, Mar. 03, 2005 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. G01N 27/327 (2006.01) | ||
| U.S. Cl. 205—777.5 [205/792] | 47 Claims |
| 1. A method of detecting nucleic acids each having a predetermined sequence included in a test liquid, the method comprising:
contacting the test liquid to a nucleic acid detection sensor, the nucleic acid detection sensor comprising: a plurality of
nucleic acid chain fixed electrodes to each of which a probe nucleic acid chain is fixed; a counter electrode which is arranged
opposite to the nucleic acid chain fixed electrodes, in which a current flows between the counter electrode and each nucleic
acid chain fixed electrode; and a reference electrode, in which the reference electrode and the nucleic acid chain fixed electrodes
are formed on the same plane and the reference electrode is formed so as to surround the nucleic acid chain fixed electrode;
performing a hybridization reaction between the probe nucleic acid chains and nucleic acid chains in the test liquid; and
measuring electrochemical signals flowing between the plurality of nucleic acid chain fixed electrodes and the counter electrode.
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