	US 7,491,304 B2
	Carrierless electrophoresis process and electrophoresis device for carrying out this process
Gerhard Weber, Kirchheim (Germany)
Assigned to Becton, Dickinson and Company, Franklin Lakes, N.J. (US)
Appl. No. 10/450,829 PCT Filed Dec. 07, 2001, PCT No. PCT/EP01/14408 § 371(c)(1), (2), (4) Date Jun. 18, 2003, PCT Pub. No. WO02/50524, PCT Pub. Date Jun. 27, 2002.
Claims priority of application No. 100 63 097 (DE), filed on Dec. 18, 2000.
Prior Publication US 2004/0045826 A1, Mar. 11, 2004
Int. Cl. G01N 27/447 (2006.01)

U.S. Cl. 204—450 [204/600]

8 Claims

1. A carrierless electrophoresis method, comprising the steps of:

(a) carrying out a coarse fractionation of a sample substance by carrierless electrophoresis in a first separation space as a first stage;

(b) carrying out a fine fractionation of a targeted portion of the coarsely fractionated sample substances by carrierless electrophoresis in a second separation space as a second stage, wherein a smaller number of fractionation sites are used in step (a) than in step (b) wherein the first and second separation spaces are adjacent to one another and comprise at least one electrode positioned between the first and second separation spaces; and