	US 7,488,467 B2
	High throughput genetic screening of lipid and cholesterol processing using fluorescent compounds
Steven Farber, Cherry Hill, N.J. (US); Michael Pack, Philadelphia, Pa. (US); and Marnie Halpern, Pikesville, Md. (US)
Assigned to Trustees of the University of Pennsylvania, Philadelphia, Pa. (US); and Carnegie Institute of Washington, Washington, D.C. (US)
Filed on Jan. 13, 2003, as Appl. No. 10/341,538.
Application 10/341538 is a continuation of application No. 09/974550, filed on Oct. 10, 2001, abandoned.
Claims priority of provisional application 60/264977, filed on Jan. 30, 2001.
Claims priority of provisional application 60/238928, filed on Oct. 10, 2000.
Prior Publication US 2003/0135869 A1, Jul. 17, 2003
Int. Cl. A61K 49/00 (2006.01)

U.S. Cl. 424—9.2 [424/1.11; 424/9.1]

21 Claims

1. A method of assaying lipid or cholesterol metabolism, or a combination thereof, in a vertebrate's digestive system by a high through-put assay in a teleost. the method comprising:

providing fluorescently labeled , quenched or unquenched lipid reporters, wherein fluorescent moieties are covalently linked to the lipid and/or cholesterol being assayed, such that the resulting lipid reporters are absorbed by the vertebrate's digestive system or function as substrates for target enzymes in the vertebrate's digestive system:

bathing the teleosts in media comprising the labeled lipid reporters, such that the lipid reporters are ingested by the teleost into its digestive system:

screening for and detecting accumulated fluorescence in digestive organs of the teleost as an indicator of metabolic processing or uptake of the lipid reporter in the digestive system: and

quantifying the amount and rate of fluorescence from the processed or unprocessed fluorescently labeled lipid reporters in the digestive organs of the teleost, providing a high through-put readout of digestive organ function, physiology of the digestive system and organs, and metabolic lipid processing.