| US 7,485,441 B2 | ||
| Chimeric DNA-binding proteins | ||
| Joel L. Pomerantz, Cambridge, Mass. (US); Phillip A. Sharp, Newton, Mass. (US); and Carl O. Pabo, Newton, Mass. (US) | ||
| Assigned to Massachusetts Institute of Technology, Cambridge, Mass. (US) | ||
| Filed on Mar. 07, 2006, as Appl. No. 11/369,889. | ||
| Application 09/852370 is a division of application No. 08/973131, granted, now 6,326,166, previously published as PCT/US95/16982, filed on Mar. 16, 1998. | ||
| Application 11/369889 is a continuation of application No. 09/852370, filed on May 10, 2001, granted, now 7,008,780. | ||
| Prior Publication US 2007/0150973 A1, Jun. 28, 2007 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. C12P 21/02 (2006.01) | ||
| U.S. Cl. 435—69.7 [435/325; 435/455] | 28 Claims |
| 1. A method for genetically engineering a cell to regulate the expression of a target gene, the method comprising introducing
into the cell a regulatably expressible nucleic acid encoding a fusion protein comprising a transcription regulatory domain
and a composite DNA binding domain, wherein the composite DNA binding domain contains one or more zinc finger domains and:
(a) binds to the target gene, and
(b) contains at least two nucleic acid-binding domains which:
(i) do not occur in the same protein in nature,
(ii) do not occur in the same protein in the order in which they are present in the composite DNA binding domain, or
(iii) do not occur in nature with the same spacing that is present in the composite DNA binding domain.
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