US 7,485,303 B2
Pharmaceutical composition of F(ab')2 antibody fragments
Juan Lopez de Silanes, Mexico (Mexico); Rita G Mancilla-Nava, Mexico (Mexico); and Jorge F Paniagua-Solis, Mexico (Mexico)
Assigned to Instituto Bioclon, S.A. de C.V., México, D.F. (Mexico)
Filed on Oct. 23, 2003, as Appl. No. 10/690,639.
Application 10/690639 is a division of application No. 09/798076, filed on Mar. 05, 2001, granted, now 6,709,655.
Prior Publication US 2004/0166107 A1, Aug. 26, 2004
Int. Cl. A61K 39/395 (2006.01); C07K 1/30 (2006.01)
U.S. Cl. 424—158.1  [424/133.1; 424/177.1; 530/387.1] 12 Claims
 
1. A pharmaceutical composition comprising polyclonal F(ab′)2 antibody fragments substantially free from albumin and whole antibodies and substantially free of pyrogens, wherein said composition binds and neutralizes a purified antigenic molecule or mixture of antigenic molecules found in the venom of a scorpion of the species Centruroides limpidus, and wherein the F(ab′)2 antibody fragments are obtained by the method which comprises:
(a) contacting a source of antibody with pepsin under conditions to prepare an antibody digest containing F(ab′)2 fragments and being substantially free of unhydrolyzed antibodies;
(b) treating said antibody digest by two steps of ammonium sulfate precipitation, i) one step at about 16% to about 22% weight by volume ammonium sulfate; and ii) another step at about 32% to about 38% weight by volume of ammonium sulfate.