| US 7,482,173 B2 | ||
| Method for attachment of silylated molecules to glass surfaces | ||
| Viswanadham Garimella, Vernon Hills, Ill. (US); and Yasmith Bernal, Lake Zurich, Ill. (US) | ||
| Assigned to Nanosphere, Inc., Northbrook, Ill. (US) | ||
| Filed on Jan. 19, 2007, as Appl. No. 11/656,312. | ||
| Application 11/656312 is a division of application No. 10/447073, filed on May 28, 2003, granted, now 7,297,553. | ||
| Claims priority of provisional application 60/383564, filed on May 28, 2002. | ||
| Prior Publication US 2008/0097115 A1, Apr. 24, 2008 | ||
| Int. Cl. G01N 33/543 (2006.01); G01N 33/551 (2006.01); G01N 33/552 (2006.01); G01N 33/00 (2006.01); C12Q 1/00 (2006.01); C12Q 1/68 (2006.01); C12N 11/00 (2006.01) | ||
| U.S. Cl. 436—518 [436/524; 436/527; 436/106; 436/109; 436/127; 435/4; 435/6; 435/174] | 6 Claims |

| 1. A compound having a formula vii:
[(R1)(R2)(R3)Si—X-Z-CYNH]2Si (NHCYL-M)2 vii
wherein R1, R2 and R3 independently represents C1-C6 alkoxy, C1-C6 alkyl, phenyl, or aryl substituted with one or more groups selected from the group consisting of C1-C6 alkyl and C1-C6 alkoxy; L represents a linking group; X represents linear or branched C1-C20 alkyl or aryl substituted with one or more groups selected from the group consisting of C1-C6 alkyl and C1-C6 alkoxy, optionally substituted with one or more heteroatoms comprising oxygen, nitrogen, or sulfur; Y represents oxygen or
sulfur; and Z represents oxygen or NH; and M represents a molecule, with the proviso that at least one of R1, R2, or R3 represents C1-C6 alkoxy.
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