| US 7,482,162 B2 | ||
| Determination of vitamin D metabolite and displacement from plasma or serum binding proteins | ||
| David Laurie, Wylam (United Kingdom); Alexander Kirkley Barnes, Gateshead (United Kingdom); and Michael James Gardner, Newcastle (United Kingdom) | ||
| Assigned to Immunodiagnostic Systems Ltd., (United Kingdom) | ||
| Appl. No. 10/433,923 PCT Filed Dec. 06, 2001, PCT No. PCT/GB01/05395 § 371(c)(1), (2), (4) Date Dec. 17, 2003, PCT Pub. No. WO02/46746, PCT Pub. Date Jun. 13, 2002. |
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| Claims priority of application No. 0029729.1 (GB), filed on Dec. 06, 2000. | ||
| Prior Publication US 2004/0096900 A1, May 20, 2004 | ||
| Int. Cl. G01N 33/49 (2006.01); G01N 33/02 (2006.01) | ||
| U.S. Cl. 436—20 [424/530; 424/531; 426/425; 426/429; 435/7.72; 435/7.9; 435/7.92; 435/188; 436/18; 436/71; 436/174; 436/177; 436/178; 436/817; 436/825; 436/826; 516/113; 516/139; 516/141; 516/143; 516/146; 516/153; 530/402; 530/412; 530/422; 530/423; 530/424] | 12 Claims |
| 1. A method for measuring vitamin D metabolite present in a plasma or serum sample, the method comprising:
(a) adding to a serum or plasma sample a non-competitive displacement agent comprising 8-anilino-1-napthalenesulfonic acid
ammonium salt, 3-(acetonylbenzyl)-4-hydroxycoumarin and a water miscible solvent, which separates any vitamin D metabolite
in the sample from protein to which the vitamin D metabolite is bound, such that any separated vitamin D metabolite can be
captured;
(b) contacting the sample with a support having immobilised thereon a binding factor, the binding factor capturing any vitamin
D metabolite separated in step (a); and
(c) measuring the amount of vitamin D metabolite captured in step (b);
wherein capture of separated vitamin D metabolite occurs in step (b) without prior extraction from the sample of the protein
separated from the vitamin D metabolite in step (a).
|