US 7,482,159 B2
	Snail, a new marker for tumour invasion and target protein of new antitumoral compounds
Amparo Cano García, Madrid (Spain); M. Angela Nieto Toledano, Madrid (Spain); M. Isabel Rodrigo Castro, Madrid (Spain); Annamaria Locascio, Madrid (Spain); Marta García del Barrio, Madrid (Spain); María José Blanco Fernandez de Valderrama, Madrid (Spain); Francisco Portillo Pérez, Madrid (Spain); and Mirna Alicia Pérez Moreno, Madrid (Spain)
Assigned to Consejo Superior de Investigaciones Cientificas, Madrid (Spain); and Universidad Autonoma de Madrid Ciudad Universitaria de Cantoblanco Crta, Madrid (Spain)
Filed on Nov. 15, 2006, as Appl. No. 11/599,664.
Application 11/599664 is a division of application No. 10/461256, filed on Jun. 13, 2003.
Application 10/461256 is a division of application No. 09/806445, granted, now 6,617,121, previously published as PCT/ES00/00226, filed on Jun. 27, 2000.
Claims priority of application No. 9901466 (ES), filed on Jul. 01, 1999.
Prior Publication US 2007/0065450 A1, Mar. 22, 2007
Int. Cl. C12N 15/85 (2006.01)

U.S. Cl. 435—455

4 Claims

1. A method of altering the invasive and metastatic capacity of isolated epithelial cells said method comprising transfecting said cells with an expression vector comprising a polynucleotide encoding a full-length Snail, protein or a partial sequence thereof binding to E-cadherin promoter, thereby up-regulating expression of Snail in the cells, and up-regulation of said expression of Snail, by said polynucleotide causing an alteration of the invasive and metastatic capacity of said cells.