US 7,482,013 B2
Albumin fusion proteins
David J. Ballance, Berwyn, Pa. (US); Darrell Sleep, West Bridgford (United Kingdom); Christopher P. Prior, Rosemont, Pa. (US); Homayoun Sadeghi, Doylestown, Pa. (US); and Andrew J. Turner, King of Prussia, Pa. (US)
Assigned to Human Genome Sciences, Inc., Rockville, Md. (US); and Delta Biotechnology Limited, Nottingham (United Kingdom)
Filed on Mar. 14, 2005, as Appl. No. 11/78,914.
Application 11/078914 is a continuation of application No. 09/832501, filed on Apr. 12, 2001, abandoned.
Claims priority of provisional application 60/256931, filed on Dec. 21, 2000.
Claims priority of provisional application 60/199384, filed on Apr. 25, 2000.
Claims priority of provisional application 60/229358, filed on Apr. 12, 2000.
Prior Publication US 2005/0266533 A1, Dec. 01, 2005
Int. Cl. A61K 39/00 (2006.01)
U.S. Cl. 424—192.1  [435/7.1; 435/69.7; 530/350] 21 Claims
 
1. An albumin fusion protein comprising a serum cholinesterase polypeptide fused to an albumin polypeptide, wherein said serum cholinesterase polypeptide is selected from:
(a) a full-length serum cholinesterase;
(b) a mature serum cholinesterase; and
(c) a fragment of serum cholinesterase, wherein said fragment has serum cholinesterase activity;
and wherein said albumin polypeptide is selected from:
(i) a full-length albumin;
(ii) a mature albumin;
(iii) a fragment of albumin, wherein said fragment of albumin has the ability to prolong the serum half-life of the fused serum cholinesterase polypeptide compared to the unfused serum cholinesterase polypeptide;
(iv) a polypeptide consisting of an amino acid sequence at least 95% identical to SEQ ID NO:18, wherein said polypeptide has the ability to prolong the serum half-life of the fused serum cholinesterase polypeptide compared to the unfused serum cholinesterase polypeptide;
(v) amino acids 1 to 585 of SEQ ID NO:18; and
(vi) amino acids 1 to 387 of SEQ ID NO:18.