US 7,476,733 B2
	Development of a real-time PCR assay for detection of pneumococcal DNA and diagnosis of pneumococccal disease
Maria da Gloria Carvalho, Decatur, Ga. (US); Jacquelyn S. Sampson, College Park, Ga. (US); Edwin W. Ades, Atlanta, Ga. (US); George Carlone, Stone Mountain, Ga. (US); and Karen McCaustland, Snellville, Ga. (US)
Assigned to The United States of America as represented by the Department of Health and Human Services, Washington, D.C. (US)
Filed on Mar. 25, 2005, as Appl. No. 11/89,938.
Prior Publication US 2006/0216720 A1, Sep. 28, 2006
Int. Cl. C07H 21/04 (2006.01)

U.S. Cl. 536—24.3  [536/24.32]

19 Claims

1. An oligonucleotide primer for the amplification of a Streptococcus pneumoniae psa nucleic acid, wherein the oligonucleotide primer is 15 to 30 nucleotides in length and hybridizes, under conditions suitable for a polymerase chain reaction, with the nucleic acid sequence according to SEQ ID NO: 4, wherein the oligonucleotide primer comprises at least 15 consecutive nucleotides of the nucleic acid sequence according to SEQ ID NO: 1, and wherein the oligonucleotide primer is capable of directing the amplification of the Streptococcus pneumoniae psa nucleic acid.