| US 7,476,722 B2 | ||
| Methods for purifying protein | ||
| Ganesh Vedantham, Seattle, Wash. (US); Clayton Brooks, III, Bainbridge Island, Wash. (US); Joanne M Reeder, Bainbridge Island, Wash. (US); and Andrew M Goetze, Sammamish, Wash. (US) | ||
| Assigned to Immunex Corporation, Thousand Oaks, Calif. (US) | ||
| Filed on Sep. 13, 2006, as Appl. No. 11/520,921. | ||
| Application 11/520921 is a continuation of application No. 10/327495, filed on Dec. 20, 2002, granted, now 7,122,641. | ||
| Claims priority of provisional application 60/343363, filed on Dec. 21, 2001. | ||
| Claims priority of provisional application 60/347189, filed on Jan. 08, 2002. | ||
| Claims priority of provisional application 60/364272, filed on Mar. 12, 2002. | ||
| Prior Publication US 2007/0010661 A1, Jan. 11, 2007 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. A61K 39/395 (2006.01); C07K 16/00 (2006.01); C07K 16/46 (2006.01); C07K 1/16 (2006.01); C07K 1/22 (2006.01) | ||
| U.S. Cl. 530—387.3 [424/134.1; 424/176.1; 530/390.1; 530/413; 530/415] | 26 Claims |
| 1. A method for purifying a TNFR:FC protein from a sample comprising the TNFR:FC protein and at least one protein contaminant, the method comprising subjecting the sample to hydroxyapatite chromatography
in a solution,
wherein the TNFR:FC protein is separated from at least one protein contaminant by hydroxyapatite chromatography in a solution in which hydroxyapatite
chromatography is performed,
wherein the TNFR portion of the TNFR:FC protein comprises an extracellular region of a human TNFR of approximately 80 kilodaltons, and
wherein the majority of molecules of the TNFR:FC protein are recovered in a flow through and wash fraction.
|