| US 7,476,529 B2 | ||
| Altering metabolism in biological processes | ||
| Carl W. Podella, Irvine, Calif. (US); and Nils Siegfried Hauptmann, Orange, Calif. (US) | ||
| Assigned to Advanced BioCatalytics Corporation, Irvine, Calif. (US) | ||
| Filed on Mar. 11, 2004, as Appl. No. 10/799,529. | ||
| Claims priority of provisional application 60/454171, filed on Mar. 11, 2003. | ||
| Claims priority of provisional application 60/454178, filed on Mar. 11, 2003. | ||
| Prior Publication US 2004/0180411 A1, Sep. 16, 2004 | ||
| Int. Cl. C12N 1/38 (2006.01); C12P 21/00 (2006.01); C12N 1/20 (2006.01); C12N 1/14 (2006.01) | ||
| U.S. Cl. 435—244 [435/71.1; 435/252.1; 435/255.1] | 36 Claims |
| 1. A method for accelerating nutrient uptake in bacteria or yeast without a substantially commensurate increase of biomass,
comprising contacting said bacteria or yeast with a mixture of an aerobic yeast fermentation supernatant and a surface-active
agent, whereby the nutrient uptake in said bacteria or yeast is increased without a substantially commensurate increase of
biomass,
wherein the mixture of the aerobic yeast fermentation supernatant and the surface-active agent is obtained by:
fermenting under aerobic conditions a plurality of yeast cells in the presence of a nutrient source,
heating the plurality of yeast cells after the fermenting step, wherein said heating step comprises increasing the temperature
of said plurality of yeast cells to between about 40° to about 60° C. for about 2 to about 24 hours, followed by cooling to
less than 25° C.,
disrupting the cellular structure of some of the plurality of yeast cells to obtain a fermentation product,
centrifuging the fermentation product to obtain the aerobic fermentation supernatant containing peptides, and
combining the aerobic fermentation supernatant with the surface-active agent.
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