US 7,473,550 B2
Use of acacia gum to isolate and preserve a biological receptor on a biosensor
Vitaly J. Vodyanoy, Auburn, Ala. (US); James M. Barbaree, Dadeville, Ala. (US); Bryan A. Chin, Auburn, Ala. (US); William Charles Neely, Auburn, Ala. (US); Suram T. Pathirana, Sunnyvale, Calif. (US); and Timothy D. Braden, Auburn, Ala. (US)
Assigned to Auburn University, Auburn, Ala. (US)
Filed on Mar. 07, 2005, as Appl. No. 11/73,963.
Application 11/073963 is a division of application No. 10/000727, filed on Nov. 30, 2001, granted, now 7,022,514, filed on Apr. 04, 2006.
Claims priority of provisional application 60/250798, filed on Dec. 01, 2000.
Claims priority of provisional application 60/250799, filed on Dec. 01, 2000.
Prior Publication US 2005/0148019 A1, Jul. 07, 2005
Int. Cl. C12M 1/34 (2006.01); C12Q 1/68 (2006.01); G01N 33/53 (2006.01)
U.S. Cl. 435—287.2  [435/6; 435/7.1] 10 Claims
 
1. A method of preparing a biosensor for an analyte, the biosensor comprising: a biological receptor for detecting the analyte; an interface; and an electrical signal transducer; the method comprising:
(a) depositing the biological receptor on the interface, wherein the interface binds the biological receptor;
(b) covering the biological receptor with a film of Acacia gum solution; and
(c) curing the Acacia gum to form a solid disposed upon the interface, wherein the solid is dissolved by irrigating with an aqueous solution and the biological receptor remains bound to the interface.